Description
The Eagle Biosciences Human Interleukin 21 (IL-21) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-21 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-21 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-21 is added to the wells and binds to the combination of capture antibody- IL-21 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-21 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-21 standard dilutions and IL-21 sample concentration determined